In vitro toxicity evaluation of food and agricultural related nanoparticles on human primary and established cells


General Information

BIO-981-10

In vitro toxicity evaluation of food and agricultural related nanoparticles on human primary and established cells

Medical And Health

Environmental Health

This project contributes to clarifying the several important status of basic human health and environment through development of new ideas for acute/chronic toxic nano-materials, identification of nano-materials as contaminants, and processes in the food and agricultural products. The ubiquitous nature of nano-materials usage with minimal precautions has resulted in contamination of food, the workplace, and the environment. Particularly, nano-scaled products are now found in agricultural chemicals, processed foods, food packaging and food contact materials including food storage containers, plates, chopping boards etc., There are unanswered questions about the impacts of nanomaterials and/or nanoproducts on human health and the environment such as susceptibility (children, athmatics, allergics, etc) and systemic toxicological fate. NPs can be more chemically reactive and more bioactive than larger particles. Because of their very small size, NPs also have much greater access to our bodies, so they are more likely than larger particles to enter cells, tissues and organs. Moreover, there is a serious lack of information concerning the impact of food and agricultural related NPs on cell specific toxicity. This project generally supports the goals of the NSTIP in the identified strategic technology area which ensure that societal benefits. As well as, these studies generate toxicological key data, which will raise the attention and awareness for increased environmental protection against nanoparticles, as well as to facilitate new legislation on nanoparticles / chemical safety.

1. To study the pre-biological characterization of active or unbound nanoparticles particles of less than 200nm in size which are involving in the food and agricultural sectors. 2. To find their in vitro toxicity or cell viability (dosimetry analysis) and cell stress of a few chosen cell models (i.e. Lung – IMR90, WI-38; Colon – CCD-18 co and CCD841 CON; kidney – HEK 293; skin -Detroit 573, Liver – THLE-2; B-lymp – RPMI1788, hESC, mMSC. Primary foreskin fibroblast, adult hMSC, total WBC and its subpopulations (CD3, CD4, CD8, CD43, NK cells, CD16 etc) induced by food and agricultural related NPs. 3. To study the effect of NPs size, shape, and concentrations specific cellular uptake, phenotypic (morphological alteration), the plasma membrane changes, cell cycle analysis and immunological response in few chosen cell models. 4. To assess the characteristic ROS production / generation upon insult by foreign materials (NPs) produces short-lived highly and/or chemically reactive oxygen/nitrogen radicals of several types, often damaging the cells through lipid peroxidation pathways. 5. Where there is actively uptake foreign particles, to find a). the response of signalling cascades; if there is an indication of cell death, to find (b) the expression of certain crucial cell death markers including apoptosis / necroapoptosis / necrosis.

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